General Cell Based Assays
Abcam Senescence Detection Kit
Senescence Detection Kit
The product is subject to the following: Abcam Restricted Use Statement
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-NF-κB (Ser536) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-NF-κB (Ser536) Detection Kit, Control Lysate
ILEX LIFE SCIENCES LLC Biocolor Sircol Soluble/Insoluble Collagen Assay Kits Combination Pack (110 Assays Soluble Collagen, 110 Assays Insoluble Collagen)
Biocolor Sircol™ Soluble Collagen Assay is a quantitative assay for measurement both acid-soluble and pepsin-soluble collagens. The colorimetric assay is a dye-binding method using Sircol™ Dye Reagent, containing Sirius Red dye in picric acid. Kit is suitable for all mammalian collagens, Types I to V. The Sircol™ Assay can monitor collagen produced in situ, or during in-vitro cell culture. Can also measure in-vitro extracellular matrix (ECM) formation. The Biocolor Sircol™ INSOLUBLE Collagen Assay is a dye-binding method for the analysis of insoluble collagen fibers. Uses Sircol™ Dye Reagent, containing Sirius Red dye in picric acid. Replaces the conventional hydroxyproline analysis. No more working with concentrated acids or alkalis! This assay uses mild acid & temp. treatment for 2-3 hrs. Solubility conversion to denatured collagen & subsequent measurement can be completed within 1 work day. Sources of material includes tissues, bone & calcified tissue.
ILEX LIFE SCIENCES LLC Immuchrom Bile Acids Assay for Human Stool Samples, Photometric Assay Kit, 96 wells
The Immuchrom Bile Acids Assay Kit is photometric test that enables easy, rapid and precise quantitative determination of free bile acids in human stool samples. Calibration Range: 9 - 280 umol/L. Detection Limit: 1.91 umol/L. Principle: The quantitative determination of bile acids is carried out via their enzymatic oxidation to 3-keto steroids with the formation of thio-NADH. The resulting change in absorbance at 405 nm is determined photometrically. The concentration can be calculated from the standard curve. Application: If the absorption of bile acids in the small intestine is impaired, they are increasingly excreted in the stool, which leads to irritation of the colon and causes severe diarrhea, resulting in severe fluid loss as well as vitamin and mineral deficiencies. This is called bile acid malabsorption syndrome or chologenic diarrhea. Synonyms: steroid carboxylic acids, cholic acid, chenodeoxycholic acid.
Cayman Chemical Acetyl-Coenzyme A-d3sodIum 1mg
An internal standard for the quantification of acetyl-coenzyme A by GC- or LC-MS
Eurofins Discoverx PathHunter MRGPRX2 Bioassay Kit, 10-Plate. (93-0309Y2-00144)
The PathHunter MRGPRX2 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility, and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Meso Scale Discovery R-PLEX Human IL-32 ASY
The R-PLEX Human IL-32 Assay Kit from Meso Scale Discovery measures IL-32 in human serum plasma and cell culture supernatants using electrochemiluminescence detection It offers high sensitivity a 5-log dynamic range and low sample volume needs The kit includes a 96-well plate with IL-32 capture antibodies detection antibodies buffers diluent and calibrators The assay takes about 3 5 hours and requires a MESO QuickPlex SQ 120 instrument It enables accurate quantitation of IL-32 an inflammatory cytokine in immune responses
Eurofins Discoverx PathHunter Human PRLHR β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PRLHR β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PRLHR (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human MIP-1 alpha (CCR1) Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human MIP-1 alpha (CCR1) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CCR1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse LTB4R β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse LTB4R β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mLTB4R1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Abcam Maleimide Quantification Assay Kit (Colorimetric).
Maleimide Quantification Assay Kit (Colorimetric).
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress PTGFR U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0936E3CP5L)
The PathHunter PTGFR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. cloprostenol) based activation of PTGFR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGFR and EA-tagged β-Arrestin-2. Activation of PTGFR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Culture Kit-112, (92-3112G)
AssayComplete Cell Culture Kit-112 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
AnaSpec SensoLyte Red Glucocerebrosidase (GBA) Assay Kit *Fluorimetric*
The SensoLyte Red Glucocerebrosidase (GBA) Assay Kit detects GBA activity by using a highly sensitive fluorogenic substrate. The GBA substrate provided in the kit releases the red fluorescent dye resorufin upon Glucocerebrosidase cleavage with absorption/emission maxima at 570 nm/610 nm. This assay kit can be used to detect enzyme activity in purified enzyme preparations and compound screening. The Red Glucocerebrosidase (GBA) Assay is one-step homogenous reaction, which does not require the additional step of dispensing stop solution after the incubation, thereby suitable for HTS. The long wavelength fluorescence resorufin is also less interfered by the autofluorescence of components in biological samples and test compounds.
Cayman Chemical GlycolysIs Cel-Based asy K 1ea
Cayman’s Glycolysis Cell-Based Assay Kit provides a colorimetric method for detecting L-lactate, the end product of glycolysis, produced and secreted by cultured cells. In this assay, lactate dehydrogenase (LDH) catalyzes the reaction between NAD+ and lactate, yielding pyruvate and NADH. The NADH directly reduces a tetrazolium salt (INT) to a colored formazan which absorbs between 490 and 520 nm. The quantity of formazan produced is proportional to the quantity of lactate in the culture medium and is thus an indirect measurement of glycolysis. This assay can be adapted to high-throughput screening to examine glycolytic regulators in a cell-based system. In addition, the non-invasive sampling leaves the cells intact and thus allows multiplex testing for additional markers.